Regulatory

Part:BBa_K3612012:Design

Designed by: Jesus Durand, Matias Rojas, Diego Benites, Maria Castromonte   Group: iGEM20_UPCH_Peru   (2020-10-23)


Pseudoalteromonas inducible promoter (pSHAB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1757
    Illegal AgeI site found at 2129
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence optimized for Pseudoalteromonas nigrifaciens.


Source

Part of the chromosome II of Pseudoalteromonas haloplanktis TAC125 (from 423068 to 419631 bp). Accession: CR954247.

References

(1) Sannino F, Giuliani M, Salvatore U, Apuzzo GA, de Pascale D, Fani R, et al. A novel synthetic medium and expression system for subzero growth and recombinant protein production in Pseudoalteromonas haloplanktis TAC125. Appl Microbiol Biotechnol. 2017 Jan 27;101(2):725–34.

(2) Parrilli E, Tutino ML. Psychrophiles: From Biodiversity to Biotechnology: Second Edition. Psychrophiles From Biodivers to Biotechnol Second Ed. 2017;1–685.

(3) Papa R, Rippa V, Sannia G, Marino G, Duilio A. An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125. J Biotechnol. 2007 Jan 1;127(2):199–210.

(4) Yu ZC, Tang BL, Zhao DL, Pang X, Qin QL, Zhou BC, et al. Development of a cold-adapted Pseudoalteromonas expression system for the Pseudoalteromonas proteins intractable for the Escherichia coli system. PLoS One. 2015;10(9):1–14.

(5) Papa, R., Glagla, S., Danchin, A. et al. Proteomic identification of a two-component regulatory system in Pseudoalteromonas haloplanktis TAC125. Extremophiles. 2006; 10,483–491.

(6) Papa, R., Rippa, V., & Duilio, A. Identification of the transcription factor responsible for l-malate-dependent regulation in the marine Antarctic bacteriumPseudoalteromonas haloplanktis TAC125. FEMS Microbiology Letters. 2009;295(2), 177–186